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Similarly, in vitro binding assay with recombinant GSTO1 was performed using 1 M GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 M DTT) incubated with compounds for 30 min at 37 C and with CMFDA (500 nM) for an additional 30 min

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Plenty of water should be consumed along with high fiber food, and hemorrhoids may relapse due to straining or extended time in toilet.

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Vitamin B12 deficiency: A deficiency in vitamin B12 can lead to severe oxidative stress Consumption of certain foods, especially fried, refined and processed foods, trans fats, artificial sweeteners, food containing carcinogens, and certain dyes and additives

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