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The qPCR was performed with the following program: 95C for 5 min of initial denaturation, then 40 cycles of 95C for 30 s, 60C for 30 s and 72C for 10 s with fluorescence acquirement, followed by a final melting curve step
It's possible that, in the future, we'll learn something that makes it either a much better or much worse option than initially assumed
Endocrinology 153:43544366
Acta Neurol Taiwan 2005;14:4854 Talaei A, Siavash M, Majidi H, Chehrei A
Continuous data was calculated using either the student's t -test or Mann Whitney U -test
However, the frequency may vary depending on your individual health needs and goals
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