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2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

Most amino acid sequence work demands 95%+ purity to avoid confounding variables

311 (Pt 2), 425430 (1995)

TB-500 is a 43-amino-acid fragment of Thymosin Beta-4 that promotes wound healing through actin sequestration regulating the cytoskeletal dynamics that drive cell migration, differentiation, and tissue repair

(5) All sources used are properly disclosed (correct citation)

The discoloration itself isn't dangerous