Further studies are required to decipher the complex regulation of expression of different isoforms of the AsA-GSH pathway enzymes in order to bolster our understanding of ROS homeostasis in plants
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For kinetic measurements, 0.2 M roGFP2 were incubated with buffer alone, a 20-fold molar excess of oxidized DsbASP, GSSG or both in a 96-well plate (Nunc, Rochester, NY)
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
Redox regulation of cell growth and cell death
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