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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

We do recommend you wait at least 2 weeks after your initial injection to make any decision about dissolving, since swelling can mimic bumpy filler for at least the first few weeks

When a patient asks about DSIP for sleep, the responsible answer is some version of: the evidence is genuinely weak, so let's make sure we've exhausted the validated approaches first

Pilar Muiz PhD

10.3389/fcell.2021.728172 546 ZhangN.YuX.XieJ.XuH