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C.PeestU.BeutelG.TholF.LevkauB.et al (2010)
cancer cells (A549) as well as non cancer cells (3T3-L1, 13S24), showed a dose-dependent decrease of cell index in response to increasing concentrations of reduced glutathione, although the reduction of cell index was more or less marked according to cell line
Briefly, the assay was performed in 500 l of 50 mM Tris-HCL, 0.1 mM EDTA, pH 7.4 containing 1 IU of glutathione-reductase (10105678001, Sigma-Aldrich), 0.5 mM GSH (G6529, Sigma-Aldrich), 0.12 mM nicotinamide adenine dinucleotide phosphate (NADPH), (N6505, Sigma-Aldrich), and 0.2 mM H 2 O 2 (95321, Sigma-Aldrich) as substrate
Thyroid nodules are common and usually benign, but evaluation often includes ultrasound and blood tests to assess thyroid function and risk characteristics
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