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this pathway has been shown to impair process outgrowth in neurons and regulate glycolysis - the major source of of ATP in mature OLs which contributes to protein and lipid production needed for myelin biosynthesis
* KruskalWallis and MannWhitney U tests , ANOVA and Student's T-tests , 2 test
Age represents one clear response modifier, with some evidence suggesting greater absolute improvements in older patients with more pronounced baseline deficits in matrix density and collagen content
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Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark
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