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To further corroborate these findings, we performed immunohistochemistry to assess the expression of SOX17 and FOXA2 in the putative DE populations, we observed strong staining for both proteins with the most robust staining observed when using RPMI supplemented with B27 with insulin and 3 M CHIR99021 (see Fig
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Interestingly, subjects with higher dietary spermidine intake had greater hippocampal volume, mean cortical thickness and cortical thickness in Alzheimers-disease-vulnerable brain regions (Reference Schwarz, Horn and Benson19)
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