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Lysates were collected and centrifuged at 17,000 rpm for 5 min at 4 C to pellet cell debris and then added into HEGA buffer for High-Performance Liquid Chromatography (HPLC) analysis
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NET WT 1.0 FL OZ (30mL)
Furthermore, the authors reported that the MCF-7 breast cancer cell line shows an E2-dependent increase in its survival rate, which is abrogated by pre-treating them with the anti-estrogenic compounds ICI-182, 780, or raloxifene (Yu et al., 2012)
PMID 2781235
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