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1D ) compared to SD, with an especially large diet-induced increase in IWAT in males

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Then, tissue fragments were transferred to a conical tube with digestion buffer (PB, 2.5 mM glucose, 2.5 mg/g of Type I collagenase and 0.1% FFA-free BSA) and incubated for 20 min at 37 C on a bath shaker

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Nutritional support Protein intake: Tissue repair requires amino acid building blocks

It must be kept refrigerated and typically has a much shorter shelf life, often just a few weeks