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The resin in the column was first washed with 5 column volumes (CVs) of buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 5 M tirzepatide, 0.1% (w/v) LMNG and 0.02% (w/v) CHS
Once reconstituted, peptides should be refrigerated at 4C and used within 30 days
Depending on your specific goals, treatment plans may involve multiple infusions over several months
Plotted from data in J Am Soc Nephrol 2006
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