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The day of the assay, cells were washed in KRB-HEPES buffer (KRBH buffer: 135 mM NaCl, 3.6 mM KCl, 0.5 mM NaH 2 PO 4 , 0.5 mM MgSO 4 , 1.5 mM CaCl 2 , 2 mM NaHCO 3 , and 10 mM HEPES, pH 7.4) and 0.1% BSA, preincubated at 37 C for 30 min in KRBH-1% BSA and washed again in KRBH-0.1% BSA

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