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Angiogenesis Studies: Angiogenic potential investigated through multiple assays: HUVEC tube formation (network complexity), endothelial migration (cells/field), aortic ring sprouting (sprout length/number), VEGF/VEGFR2 expression (qPCR, Western blot), CAM assay (vessel density)
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Subcutaneous tissue thickness, regional blood flow, and injection technique all introduce variables that don't affect IM injection to the same degree
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