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The selected compounds for this assay were incubated at different concentrations (6 mg/mL, 3.0 mg/mL, 1.5 mg/mL, and 0.75 mg/mL of the samples, and 0.06 mg/mL, 0.03 mg/mL, 0.015 mg/mL and 0.0075 mg/mL of Alendronate) and incubated with 50 L of human erythrocytes resuspended in 950 L of saline solution