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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS

Glutathione is a natural sulfur-containing tripeptide composed of the amino acids L-glycine, glutamic acid, and L-cysteine

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Improved mood regulation and a noticeable reduction in depressive symptoms

The main concern over a vitamin B12 deficiency is that it can lead to anemia, a condition where there are fewer healthy red blood cells