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Spray once gently, keeping the nozzle level and avoiding direct upward aim into the sinuses
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Elution steps were performed in an according buffer (50 mM Tris-HCl, 140 mM NaCl, 2 mM DDT, 5-10 mM reduced glutathione, pH 8.0)
361, Kita No
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The walls of each open-field chamber were made of Plexiglas with a ventilated top-cover
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