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Development and characterization of a preclinical total marrow irradiation conditioning-based bone marrow transplant model for sickle cell disease
That sudden return of circulation, in effect, a shock to the system, is known to cause a surge of inflammation and oxidative stress, that can actually make things worse
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For monitoring cellular GSH levels, all MSCs were loaded with 2 M FreSHtracer for 2 h in the culture medium and subsequently sorted at 4 C according to their F 510 /F 580 levels using an AriaIII Flow Cytometer System (BD Biosciences, San Jose, CA) [8]
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