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Once cells reached mid-log phase (OD 600 = 0.40.6), they were pelleted in 50-ml conical tubes, and the supernatant was completely removed
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The sections were deparaffinized, hydrated, antigen retrieved, and then probed with primary antibodies against KIM-1, IL-1, TNF-, F4/80, 4-HNE, GPX4, Ferritin, and p53 at the dilution of 1:200, followed by the incubation with the secondary antibodies for 60min on the second day
Consultation with Healthcare Providers : As with any supplement, it is vital to consult with a healthcare provider before starting liposomal glutathione, particularly during pregnancy
The mean age was 47 years