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ALL leukemic blasts for example, lack significant expression of asparagine synthetase, causing a dependence on extracellular asparagine

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Furthermore, it has been documented that a deficiency in Mn 2+ leads to a reduction in the transcription of sodA , suggesting that the expression of this gene is controlled through an unidentified pathway that responds to Mn 2+ levels (Eijkelkamp et al., 2014)

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Cells were stained with CD19, 7-AAD, and anti-Annexin V-PE diluted 1:200 (BioLegend, 640908) for 20 min, RT

Step 3: Add Water Volume Enter the volume of bacteriostatic water (in milliliters, mL) used for mixing