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Hence, a peptide was designed by substituting the negatively charged residues D42, D48, D49, E51, E56, and D57 within the p53 TAD PEP with alanine to increase its binding affinity to FOXO4 FHD and enhance membrane penetration

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doi: 10.1016/j.bbrc.2022.04.062 56 HongJXuHYuLYuZChenXMengZet al