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Reduced or oxidized His-roGFP2 and reduced strep-MBP-GRX samples were passed through PD MiniTrap G-25 desalting columns (GE Healthcare) so that the proteins were finally in 100 mM Tris/HCl, p H 8.0, 1 mM EDTA
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3gss: AJ Oakley, J Rossjohn, M Lo Bello, AM Caccuri, G Federici & MW Parker (1997) The three-dimensional structure of the human Pi class glutathione transferase P1-1 in complex with the inhibitor ethacrynic acid and its glutathione conjugate
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