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It has been observed that repeated doses of DCA resulted in loss of GSTZ1-1 protein and activity, probably due to post-translationally modifications of the enzyme 85

Engineering of gasdermin D-cleaving proteases Although the evolved X(GD_L) proteases displayed promising activity through profiling activity in the PACE architecture, they failed to yield measurable kinetic parameters or substrate cleavage in mammalian cells, likely due to insufficient catalytic activity (Fig

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