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Extraction and enzymes assays Fresh maize leaves (1 g) were homogenized in 100 mM Tris-HCl in presence of 5 mM DTT (dithiothreitol), 10 mM MgCl 2 , 1 mM EDTA, 5 mM magnesium acetate, 1.5% polyvinylpyrrolidone (PVP-40), 1 mM phenylmethylsulfonyl fluoride, and 1 g mL 1 aprotinin
Vitamin B12 injections are often used for this condition because they get B12 into the blood without going through the digestive system
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