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Protein bands were visualized with an ECL solution (Thermo Fisher Scientific) and imaged with a ChemiDoc Imaging System (BIO-RAD)

Standard rapid-release formulas, even if absorbed, create a brief spike in blood levels followed by a rapid crash, whereas sustained-release formulations maintain a therapeutic window that provides round-the-clock cellular protection

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NAD+ participates in energy-transfer reactions and serves as a substrate for enzymes involved in cellular signaling and DNA repair