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Key Steps: Desalting to remove buffer salts that interfere with ionization Reduction and alkylation (if required) to stabilize disulfide bonds Removal of interfering substances such as excipients or contaminants Importance: Improper sample preparation can result in: Poor enzymatic digestion efficiency Signal suppression in mass spectrometry Inaccurate or misleading analytical results 2
Commentary: vitamin C: antioxidant or pro-oxidant in vivo
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