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The retentate was suspended in PBS and then re-centrifuged

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Diabetes Care 39 , 18961901 (2016)

This mechanism raises the critical discontinuation question - does stopping GHK-Cu immediately halt enhanced collagen production returning synthesis rates to pre-treatment baseline, or does the improved dermal environment created during treatment continue supporting elevated collagen production through positive feedback mechanisms where better matrix structure itself promotes ongoing synthesis even without continuous peptide stimulation