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The present work compares, for the first time, whether the season-dependent effects of heat stress (winter, spring, summer, and autumn) are replicated in an in vitro model, which is crucial for a deeper understanding of the physiological mechanisms underlying oocyte damage

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Furthermore, the specific inhibition of NNMT by 5-amino-1mq avoids interference with other metabolic enzymes, ensuring the specificity and reliability of experimental results