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Briefly, 80 l of 67 mM potassium phosphate buffer (NaH 2 PO 4 -Na 2 HPO 4 , pH6.8), 25 l of desired concentration of inhibitor solution and 125 l of 5 mM L-tyrosine were mixed and added into each well of a 96-well Elisa plate, incubated at 25C for 5 minutes