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Subsequently, the peptide solution was diluted 1:1 using 0.2% (v/v) NP-40 in PBS, and isoDTB-conjugated peptides were enriched on high-capacity streptavidin agarose beads (50 l slurry, Fisher Scientific, 10733315) in 0.1% (v/v) NP-40 in PBS for 1 h at r.t while rotating (disc rotator)
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