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For low glucose experiments cells were plated in Neurobasal-A Medium, no D-glucose, no sodium pyruvate, supplemented with 0,5 mg/ml D-Glucose, 1 mM sodium pyruvate (ThermoFisher), and the components previously described for complete NB media, and incubated at 37 and 5% CO 2 for 72 h

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Exosomes can be stored at 20F for up to six months without losing their effectiveness, ensuring theyre ready when needed most