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Where clinical suspicion remains high despite a normal serum B12, additional markers may be requested, including: Holotranscobalamin (active B12) a more specific marker of B12 status, available in some UK laboratories as an alternative or confirmatory test [11] [12] Methylmalonic acid (MMA) elevated in functional B12 deficiency

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Cell positions were tracked using image cross-correlation velocimetry as implemented in MetaMorph software version 7.8.0.0 (Molecular Devices, California, USA)

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The anti-cholinergic agents atropine and papaverine induce constipation in animals (23)