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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

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Intramuscular placement goes deeper when large muscle groups or thick connective structures sit at the center of the plan

And the only way to find it is through systematic experimentation over four to six weeks

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