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Ferroptosis deficiency attenuated TGF--induced fibrosis in HK-2 cells via GPX4 overexpression These results demonstrated that ferroptosis inhibition improved fibrotic changes in vitro and in vivo

The qPCR was performed with the following program: 95C for 5 min of initial denaturation, then 40 cycles of 95C for 30 s, 60C for 30 s and 72C for 10 s with fluorescence acquirement, followed by a final melting curve step

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