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Threonine 102, in a few species replaced by a serine, is the closest residue with around 6 from the threonine oxygen to either an oxygen on the phosphate or to a carboxyl oxygen on one of the attached fatty acids
Dicson and R
The reaction was performed at 22 C for 20 min, and the absorbance at 546 nm was measured, using NaNO 2 solution as standard
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To improve the quality and resolution of the highly flexible catalytic domains, masked local refinement with D1 symmetry imposed with a soft mask of the most central catalytic and regulatory domains (6 dilation with a soft padding of 20 ) was used
10.1016/s0378-4274(99)00021-1 114 ShahR.MargisonK.PrattD
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