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The physiology of urinary concentration: an update

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Subsequent progenies were generated by mating Gipr fl/fl male mice (no synapsin-Cre) with female homozygous floxed Gipr mice with the synapsin-Cre transgene ( Gipr Syn/ ) to produce mice with CNS KO of Gipr ( Gipr Syn/ )

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For this she required specific antibodies that could home in on and bind to the peptides, even if in minute quantities