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A positive test result (Phe greater than 150 mol/L) prompts the physician to begin a phenylalanine-restricted formula and requires a confirmatory quantitative Phe level

After 3 months, take a 2-month break before repeating

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Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail