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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)

For optimal results, the recommended dosage of B12 typically involves injections of 1 ml, 2-3 times weekly, as advised by our physicians

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However, there are significant precautions and concerns regarding its use, particularly related to tumor growth and the lack of rigorous human studies

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