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This is standard technique for sealed vials and should be done routinely, not just when you encounter resistance

Lipofectamine 2000 and siRNA were incubated separately with DMEM/F-12 without FBS for 5 min, 37 C and then the two suspensions were mixed and incubated for 20 min, 37 C

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For a 70 kg individual, this translates to approximately 1.75-28 mg per dose, though standard therapeutic protocols use significantly lower daily doses

In fact, regular B12 supplements arent always effective at replenishing your levels