Avoid Repeated freeze-thaw cycles, direct sunlight, and temperatures above 25C
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The irradiation parameters included a source-to-skin distance of 40 cm, an absorbed dose rate of 200 cGy/min, a total body irradiation duration of 392 s, and a single cumulative radiation dose of 8 Gy
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The membranes were blocked in 5% non-fat milk and incubated overnight at 4 degrees Celsius, with primary antibodies
Deficiency of vitamin B12 and its relation with neurological disorders: A review