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Evidence increasingly supports an elevated risk associated with persistent, intensive NSW, particularly when initiated early in life or combined with genetic susceptibility

Positive control: Provides an ROS positive control reagent (Rosup, 50 mg/mL) 2

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doi:10.1007/s00701-014-2129-2

Western blotting was performed as previously reported [22-24]

In summary, although this study presents senotherapy as a promising alternative for enhancing anticancer strategies, numerous aspects of its application necessitate further investigation