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Aspirate slightly to ensure the needle is not in a blood vessel

However, iron overload in patients with hemochromatosis results in excessive PS externalization and calpain activation in RBCs [83]

The glutathione levels of those with diabetes, heart disease, or arthritis were much lower

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Cells were transferred to a 1-mm gap electrocuvette and electroporated at 1,800 V, 200 and 25 F (time constants are typically above 5.0 milliseconds)