If your protocol absolutely demands long-term storage of a liquid solution, the only viable method is to aliquot it into single-use volumes before the initial freeze
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Eckert DJ, Owens RL, Kehlmann GB, et al
NSL literature pointer PMID 2031869 L-Carnitine laboratory index [PMID: 2031869] National Science Labs indexing note for PMID 2031869: review the PubMed methods, models, and limitations before citing
