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Intracellular calcium Fluo4/AM or X-Rhod-1, AM [398]
The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)
Dose and frequency
microwave power = 0.316 mW
Freeze-thaw cycles from +5 C to 40 C were maintained with a temperature accuracy of 0.2 C
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