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Subsequently, the slices were incubated with the indicated primary antibody (against METTL3 (15073-1-AP, Proteintech, 1:500), SLC7A11 (NB300-318, Novus Biologicals, 1:200), FSP1 (HPA042309, Atlas Antibodies, 1:100), HMOX1 (10701-1-AP, Proteintech, 1:200), TFR (13-6800, Invitrogen, 1:200), or 4-HNE (MAB3249, R&D Systems, 1:2000) overnight at 4 C
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After genotype analysis, TT genotypes had a diminished level of global DNA methylation compared with those with CC wild type 28
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