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Cells or organoids were solubilized in pull-down buffer (50 mM Tris pH 7.3, 150 mM NaCl, 1% Triton X-100, 0.1 mM CaCl 2 plus protease/phosphatase inhibitors as above) and centrifuged at 14,000 rpm for 10 min at 4C
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And importantly, antibody formation was not associated with any serious allergic reactions in the trials conducted to date
62 , e202313912 (2023)
Science 335, 16381643
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