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High levels of H 2 O 2 can lead to hyperoxidation of peroxidatic cysteine thiol groups to sulfinic or sulfonic acids, which render the peroxiredoxin enzymatically inactive

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The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

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