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While the RNI for maintenance is only 1.5 g daily, therapeutic oral doses typically range from: 50-150 g daily for mild deficiency prevention in at-risk groups 1,000-2,000 g daily for treating confirmed deficiency without malabsorption Some formulations contain up to 5,000 g, though there is no proven additional benefit over 1,000-2,000 g for most patients The reason for this dosage discrepancy lies in absorption efficiency
Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)
Like I said, these peptides have been around for decades
18 (7), e45
Acknowledgements The project was supported by the National Natural Science Foundation of China (81220108006) to WJ and JY
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