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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step
Laboratory research record on GLP-1S [PMID: 41348220] PubMed laboratory record discussing GLP-1S in a research context
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The result can range from mild discomfort to debilitating pain that radiates down the legs
These problems include preeclampsia (high blood pressure and protein in the urine) in the pregnant person, pregnancy loss, and congenital anomalies (commonly known as birth defects)
