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Association of renal injury with increased oxygen free radical activity and altered nitric oxide metabolism in chronic experimental hemosiderosis

2 Methods 2.1 Animals and procedures The animal experimental procedures were approved by the Animal Care and Use Committee of Oujiang Laboratory (SYXK2023-0047, OJLAB22121609), and were carried out in strict accordance with the National Institutes of Health Guidelines for the Use of Laboratory Animals

And mice were maintained on 12/12 h light/dark cycles with water and food ad libitum

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reduction of oxidative damage

Then, a scratch wound was applied in each well using a sterile pipette tip, and this time was made at 0 h