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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
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01-SWKJYCJJ17, No
What are the side effects of B12 and lipo shots
Those with a severe deficiency may require a 5000 mcg dose
Obviously, the $$$ grubbing business weasels who sell pharmaceuticals would prefer you throw them out and fork-over for "new ones"
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